New "cocktail" to reprogram adult cells


Hebrew University scientists create therapy-grade stem cells using new cocktail to reprogram adult cells

Cells created at Hebrew University's Faculty of Medicine pass the toughest pluripotency test

Jerusalem, September 16, 2014 — Researchers at the Hebrew University of Jerusalem have developed a new cocktail that is highly effective at coaxing adult cells to become quality pluripotent stem cells.

Regenerative medicine is a new and expanding area that aims to replace lost or damaged cells, tissues or organs through cellular transplantation. Because stem cells derived from human embryos can trigger ethical concerns, a good solution is reprogramming adult cells back to an embryo-like state using a combination of reprogramming factors.

The resulting cells, called induced pluripotent stem cells (iPSCs), could be used to replace those lost to damage or disease. However, scientists have discovered that the process of reprogramming adult cells can introduce genetic abnormalities that limit the cells' usefulness in research and medicine.

To make iPSCs, scientists expose adult cells to a cocktail of genes that are active in embryonic stem cells. iPSCs can then be coaxed to differentiate into other cell types such as nerve or muscle. However, the standard combination of factors used to reprogram cells leads to a high percentage of serious genomic aberrations in the resulting cells. (The reprogramming factors are Oct4, Sox2, Klf4, and Myc — known collectively as OSKM).

Now researchers at the Hebrew University of Jerusalem have developed a new cocktail of reprogramming factors that produce high-quality iPSCs. Dr. Yosef Buganim, at the Institute for Medical Research Israel-Canada in the Hebrew University's Faculty of Medicine, worked with scientists at the lab of Whitehead Institute founding member Rudolf Jaenisch, a professor of biology at MIT.

The researchers reasoned that changing the reprogramming factors could reprogram the adult cells in a more controlled way and yield high-quality iPSCs. Working with mouse cells, Dr. Buganim and research scientist Styliani Markoulaki used bioinformatic analysis to design a new cocktail of reprogramming factors (Sall4, Nanog, Esrrb, and Lin28, known collectively as SNEL).

Their results showed that the interaction between reprogramming factors plays a crucial role in determining the quantity and quality of resulting iPSCs — and that a different combination of reprogramming factors can in fact produce a much higher quality product.

The new SNEL cocktail created fewer colonies of iPSCs, but approximately 80% of those produced passed the most stringent pluripotency test. This is highly preferable to the traditional OSKM cocktail, which produces a large number of colonies but the majority of which fail the pluripotency test.

Dr. Buganim hypothesizes that SNEL may reprogram cells better than OSKM because it does not rely on the master regulators Oct4 and Sox2, which might activate part of the adult cell genome. According to Buganim, the research demonstrates the effectiveness of bioinformatics tools in producing high quality iPSCs.

This study takes the regenerative medicine field one step closer to the clinic, where it may be able to help patients in need of cellular transplantation therapy. The researchers will now seek to define the optimal combinations for reprogramming human iPSCs, which are harder to reprogram than mouse cells and which could not be reprogrammed using the SNEL cocktail.

The research paper, "The Developmental Potential of iPSCs Is Greatly Influenced by Reprogramming Factor Selection," appears in the current issue of the journal Cell Stem Cell. Dr. Buganim was supported by a NIH Kirschstein National Research Service Award, and the research project was supported by a grant from the Israeli Centers of Research Excellence (I-CORE) program. Other researchers received support from the National Institutes of Health, National Science Foundation, Chapman Foundation, Florence Brill Graduate Student Fellowship, and Gordon and Betty Moore Foundation.